Monday, May 10, 2010

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TISSUE CULTURE

Tissue Culture Methods

I. TYPES OF CELLS GROWN IN CULTURE

Tissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often used interchangeably. Cell cultures are derived from either primary tissue explants or cell suspensions. Primary cell cultures typically will have a finite life span in culture whereas continuous cell lines are, by definition, abnormal and are often transformed cell lines.

II. WORK AREA AND EQUIPMENT

A. Laminar flow hoods. There are two types of laminar flow hoods, vertical and horizontal. The vertical hood, also known as a biology safety cabinet, is best for working with hazardous organisms since the aerosols that are generated in the hood are filtered out before they are released into the surrounding environment. Horizontal hoods are designed such that the air flows directly at the operator hence they are not useful for working with hazardous organisms but are the best protection for your cultures. Both types of hoods have continuous displacement of air that passes through a HEPA (high efficiency particle) filter that removes particulates from the air. In a vertical hood, the filtered air blows down from the top of the cabinet; in a horizontal hood, the filtered air blows out at the operator in a horizontal fashion. NOTE: these are not fume hoods and should not be used for volatile or explosive chemicals. They should also never be used for bacterial or fungal work. The hoods are equipped with a short-wave UV light that can be turned on for a few minutes to sterilize the surfaces of the hood, but be aware that only exposed surfaces will be accessible to the UV light. Do not put your hands or face near the hood when the UV light is on as the short wave light can cause skin and eye damage. The hoods should be turned on about 10-20 minutes before being used. Wipe down all surfaces with ethanol before and after each use. Keep the hood as free of clutter as possible because this will interfere with the laminar flow air pattern.

B. CO2 Incubators. The cells are grown in an atmosphere of 5-10% CO2 because the medium used is buffered with sodium bicarbonate/carbonic acid and the pH must be strictly maintained. Culture flasks should have loosened caps to allow for sufficient gas exchange. Cells should be left out of the incubator for as little time as possible and the incubator doors should not be opened for very long. The humidity must also be maintained for those cells growing in tissue culture dishes so a pan of water is kept filled at all times.

C. Microscopes. Inverted phase contrast microscopes are used for visualizing the cells. Microscopes should be kept covered and the lights turned down when not in use. Before using the microscope or whenever an objective is changed, check that the phase rings are aligned.

D. Preservation. Cells are stored in liquid nitrogen (see Section III- Preservation and storage).

E. Vessels. Anchorage dependent cells require a nontoxic, biologically inert, and optically transparent surface that will allow cells to attach and allow movement for growth. The most convenient vessels are specially-treated polystyrene plastic that are supplied sterile and are disposable. These include petri dishes, multi-well plates, microtiter plates, roller bottles, and screwcap flasks - T-25, T-75, T-150 (cm2 of surface area). Suspension cells are either shaken, stirred, or grown in vessels identical to those used for anchorage-dependent cells.

III. PRESERVATION AND STORAGE. Liquid N2 is used to preserve tissue culture cells, either in the liquid phase (-196°C) or in the vapor phase (-156°C). Freezing can be lethal to cells due to the effects of damage by ice crystals, alterations in the concentration of electrolytes, dehydration, and changes in pH. To minimize the effects of freezing, several precautions are taken. First, a cryoprotective agent which lowers the freezing point, such as glycerol or DMSO, is added. A typical freezing medium is 90% serum, 10% DMSO. In addition, it is best to use healthy cells that are growing in log phase and to replace the medium 24 hours before freezing. Also, the cells are slowly cooled from room temperature to -80°C to allow the water to move out of the cells before it freezes. The optimal rate of cooling is 1°-3°C per minute. Some labs have fancy freezing chambers to regulate the freezing at the optimal rate by periodically pulsing in liquid nitrogen. We use a low tech device called a Mr. Frosty (C#1562 -Nalgene, available from Sigma). The Mr. Frosty is filled with 200 ml of isopropanol at room temperature and the freezing vials containing the cells are placed in the container and the container is placed in the -80°C freezer. The effect of the isopropanol is to allow the tubes to come to the temperature of the freezer slowly, at about 1°C per minute. Once the container has reached -80°C (about 4 hours or, more conveniently, overnight) the vials are removed from the Mr. Frosty and immediately placed in the liquid nitrogen storage tank. Cells are stored at liquid nitrogen temperatures because the growth of ice crystals is retarded below -130°C. To maximize recovery of the cells when thawing, the cells are warmed very quickly by placing the tube directly from the liquid nitrogen container into a 37°C water bath with moderate shaking. As soon as the last ice crystal is melted, the cells are immediately diluted into prewarmed medium.

IV. MAINTENANCE

Cultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log should be maintained that is separate from your regular laboratory notebook. The log should contain: the name of the cell line, the medium components and any alterations to the standard medium, the dates on which the cells were split and/or fed, a calculation of the doubling time of the culture (this should be done at least once during the semester), and any observations relative to the morphology, etc.

A. Growth pattern. Cells will initially go through a quiescent or lag phase that depends on the cell type, the seeding density, the media components, and previous handling. The cells will then go into exponential growth where they have the highest metabolic activity. The cells will then enter into stationary phase where the number of cells is constant, this is characteristic of a confluent population (where all growth surfaces are covered).

B. Harvesting. Cells are harvested when the cells have reached a population density which suppresses growth. Ideally, cells are harvested when they are in a semi-confluent state and are still in log phase. Cells that are not passaged and are allowed to grow to a confluent state can sometime lag for a long period of time and some may never recover. It is also essential to keep your cells as happy as possible to maximize the efficiency of transformation. Most cells are passaged (or at least fed) three times a week.

1. Suspension culture. Suspension cultures are fed by dilution into fresh medium.

2. Adherent cultures. Adherent cultures that do not need to be divided can simply be fed by removing the old medium and replacing it with fresh medium.

When the cells become semi-confluent, several methods are used to remove the cells from the growing surface so that they can be diluted:

Mechanical - A rubber spatula can be used to physically remove the cells from the growth surface. This method is quick and easy but is also disruptive to the cells and may result in significant cell death. This method is best when harvesting many different samples of cells for preparing extracts, i.e., when viability is not important.
Proteolytic enzymes - Trypsin, collagenase, or pronase, usually in combination with EDTA, causes cells to detach from the growth surface. This method is fast and reliable but can damage the cell surface by digesting exposed cell surface proteins. The proteolysis reaction can be quickly terminated by the addition of complete medium containing serum
EDTA - EDTA alone can also be used to detach cells and seems to be gentler on the cells than trypsin. The standard procedure for detaching adherent cells is as follows:
1. Visually inspect daily

2. Release cells from monolayer surface


a. wash once with a buffer solution
b. treat with dissociating agent
c. observe cells under the microscope. Incubate until cells become rounded and loosen when flask is gently tapped with the side of the hand.
d. Transfer cells to a culture tube and dilute with medium containing serum.
e. Spin down cells, remove supernatant and replace with fresh medium.
f. Count the cells in a hemacytometer, and dilute as appropriate into fresh medium.

C. Media and growth requirements

1. Physiological parameters

A. temperature - 37C for cells from homeother

B. pH - 7.2-7.5 and osmolality of medium must be maintained

C. humidity is required

D. gas phase - bicarbonate conc. and CO2 tension in equilibrium
E. visible light - can have an adverse effect on cells; light induced production of toxic compounds can occur in some media; cells should be cultured in the dark and exposed to room light as little as possible;

2. Medium requirements: (often empirical)

A. Bulk ions - Na, K, Ca, Mg, Cl, P, Bicarb or CO2
B. Trace elements - iron, zinc, selenium
C. sugars - glucose is the most common
D. amino acids - 13 essential
E. vitamins - B, etc.
F. choline, inositol
G. serum - contains a large number of growth promoting activities such as buffering toxic nutrients by binding them, neutralizes trypsin and other proteases, has undefined effects on the interaction between cells and substrate, and contains peptide hormones or hormone-like growth factors that promote healthy growth.
H. antibiotics - although not required for cell growth, antibiotics are often used to control the growth of bacterial and fungal contaminants.

3. Feeding - 2-3 times/week.

4. Measurement of growth and viability. The viability of cells can be observed visually using an inverted phase contrast microscope. Live cells are phase bright; suspension cells are typically rounded and somewhat symmetrical; adherent cells will form projections when they attach to the growth surface. Viability can also be assessed using the vital dye, trypan blue, which is excluded by live cells but accumulates in dead cells. Cell numbers are determined using a hemocytometer.

V. SAFETY CONSIDERATIONS


Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should also be observed:


pipetting: use pipette aids to prevent ingestion and keep aerosols down to a minimum


no eating, drinking, or smoking


wash hands after handling cultures and before leaving the lab


decontaminate work surfaces with disinfectant (before and after)


autoclave all waste


use biological safety cabinet (laminar flow hood) when working with hazardous organisms. The cabinet protects worker by preventing airborne cells and viruses released during experimental activity from escaping the cabinet; there is an air barrier at the front opening and exhaust air is filtered with a HEPA filter make sure cabinet is not overloaded and leave exhaust grills in the front and the back clear (helps to maintain a uniform airflow)


use aseptic technique


dispose of all liquid waste after each experiment and treat with bleach

REFERENCES:

R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.

VI. TISSUE CULTURE PROCEDURES

Each student should maintain his own cells throughout the course of the experiment. These cells should be monitored daily for morphology and growth characteristics, fed every 2 to 3 days, and subcultured when necessary. A minimum of two 25 cm2 flasks should be carried for each cell line; these cells should be expanded as necessary for the transfection experiments. Each time the cells are subcultured, a viable cell count should be done, the subculture dilutions should be noted, and, after several passages, a doubling time determined. As soon as you have enough cells, several vials should be frozen away and stored in liquid N2. One vial from each freeze down should be thawed 1-2 weeks after freezing to check for viability. These frozen stocks will prove to be vital if any of your cultures become contaminated.

Procedures:1. Media preparation. Each student will be responsible for maintaining his own stock of cell culture media; the particular type of media, the sera type and concentration, and other supplements will depend on the cell line. Do not share media with you partner (or anyone else) because if a culture or a bottle of media gets contaminated, you have no back-up. Most of the media components will be purchased prepared and sterile. In general, all you need to do is sterily combine several sterile solutions. To test for sterility after adding all components, pipet several mls from each media bottle into a small sterile petri dish or culture tube and incubate at 37EC for several days. Use only media that has been sterility tested. For this reason, you must anticipate your culture needs in advance so you can prepare the reagents necessary. But, please try not to waste media. Anticipate your needs but don't make more than you need. Tissue culture reagents are very expensive; for example, bovine fetal calf serum cost ~ $200/500 ml. Some cell culture additives will be provided in a powdered form. These should be reconstituted to the appropriate concentration with double-distilled water (or medium, as appropriate) and filtered (in a sterile hood) through a 0-22 μm filter.

All media preparation and other cell culture work must be performed in a laminar flow hood. Before beginning your work, turn on blower for several minutes, wipe down all surfaces with 70% ethanol, and ethanol wash your clean hands. Use only sterile pipets, disposable test tubes and autoclaved pipet tips for cell culture. All culture vessels, test tubes, pipet tip boxes, stocks of sterile eppendorfs, etc. should be opened only in the laminar flow hood. If something is opened elsewhere in the lab by accident, you can probably assume its contaminated. If something does become contaminated, immediately discard the contaminated materials into the biohazard container and notify the instructor.

2. Growth and morphology. Visually inspect cells frequently. Cell culture is sometimes more an art than a science. Get to know what makes your cells happy. Frequent feeding is important for maintaining the pH balance of the medium and for eliminating waste products. Cells do not typically like to be too confluent so they should be subcultured when they are in a semi-confluent state. In general, mammalian cells should be handled gently. They should not be vortexed, vigorously pipetted or centrifuged at greater than 1500 g.

3. Cell feeding. Use prewarmed media and have cells out of the incubator for as little time as possible. Use 10-15 ml for T-25's, 25-35 ml for T-75's and 50-60 ml for T-150's. a. Suspension cultures. Feeding and subculturing suspension cultures are done simultaneously. About every 2-3 days, dilute the cells into fresh media. The dilution you use will depend on the density of the cells and how quickly they divide, which only you can determine. Typically 1:4 to 1:20 dilutions are appropriate for most cell lines. b. Adherent cells. About every 2-3 days, pour off old media from culture flasks and replace with fresh media. Subculture cells as described below before confluency is reached.

4. Subculturing adherent cells. When adherent cells become semi-confluent, subculture using 2 mM EDTA or trypsin/EDTA.

Trypsin-EDTA :


a. Remove medium from culture dish and wash cells in a balanced salt solution without Ca++ or Mg++. Remove the wash solution.


b. Add enough trypsin-EDTA solution to cover the bottom of the culture vessel and then pour off the excess.


c. Place culture in the 37°C incubator for 2 minutes.


d. Monitor cells under microscope. Cells are beginning to detach when they appear rounded.


e. As soon as cells are in suspension, immediately add culture medium containing serum. Wash cells once with serum containing medium and dilute as appropriate (generally 4-20 fold).

EDTA alone:


a. Prepare a 2 mM EDTA solution in a balanced salt solution (i.e., PBS without Ca++ or Mg++).


b. Remove medium from culture vessel by aspiration and wash the monolayer to remove all traces of serum. Remove salt solution by aspiration.


c. Dispense enough EDTA solution into culture vessels to completely cover the monolayer of cells.


d. The coated cells are allowed to incubate until cells detach from the surface. Progress can be checked by examination with an inverted microscope. Cells can be gently nudged by banging the side of the flask against the palm of the hand.


e. Dilute cells with fresh medium and transfer to a sterile centrifuge tube.


f. Spin cells down, remove supernatant, and resuspend in culture medium (or freezing medium if cells are to be frozen). Dilute as appropriate into culture flasks.

5. Thawing frozen cells.


a. Remove cells from frozen storage and quickly thaw in a 37°C waterbath by gently agitating vial.


b. As soon as the ice crystals melt, pipet gently into a culture flask containing prewarmed growth medium.


c. Log out cells in the "Liquid Nitrogen Freezer Log" Book.

6. Freezing cells.


a. Harvest cells as usual and wash once with complete medium.


b. Resuspend cells in complete medium and determine cell count/viability.


c. Centrifuge and resuspend in ice-cold freezing medium: 90% calf serum/10% DMSO, at 106 - 107 cells/ml. Keep cells on ice.


d. Transfer 1 ml aliquots to freezer vials on ice.


e. Place in a Mr. Frosty container that is at room temperature and that has sufficient isopropanol.


f. Place the Mr. Frosty in the -70°C freezer overnight. Note: Cells should be exposed to freezing medium for as little time as possible prior to freezing


g Next day, transfer to liquid nitrogen (DON'T FORGET) and log in the "Liquid Nitrogen Freezer Log" Book.

7. Viable cell counts. USING A HEMOCYTOMETER TO DETERMINE TOTAL CELL COUNTS AND VIABLE CELL NUMBERS (Reference: Sigma catalogue)Trypan blue is one of several stains recommended for use in dye exclusion procedures for viable cell counting. This method is based on the principle that live cells do not take up certain dyes, whereas dead cells do.

1. Prepare a cell suspension, either directly from a cell culture or from a concentrated or diluted suspension (depending on the cell density) and combine 20 μl of cells with 20 μl of trypan blue suspension (0.4%). Mix thoroughly and allow to stand for 5-15 minutes.

2. With the cover slip in place, transfer a small amount of trypan blue-cell suspension to both chambers of the hemocytometer by carefully touching the edge of the cover slip with the pipette tip and allowing each chamber to fill by capillary action. Do not overfill or underfill the chambers.3. Starting with 1 chamber of the hemocytometer, count all the cells in the 1 mm center square and four 1 mm corner square. Keep a separate count of viable and non-viable cells.4. If there are too many or too few cells to count, repeat the procedure either concentrating or diluting the original suspension as appropriate.5. The circle indicates the approximate area covered at 100X microscope magnification (10X ocular and 10X objective). Include cells on top and left touching middle line. Do not count cells touching middle line at bottom and right. Count 4 corner squares and middle square in both chambers and calculate the average.6. Each large square of the hemocytometer, with cover-slip in place, represents a total volume of 0.1 mm3 or 10-4 cm3. Since 1 cm3 is equivalent to approximately 1 ml, the total number of cells per ml will be determined using the following calculations:Cells/ml = average cell count per square x dilution factor x 104;

Total cells = cells/ml x the original volume of fluid from which the cell sample was removed; % Cell viability = total viable cells (unstained)/total cells x 100.

Monday, February 1, 2010

TOMATO PESTS

Pests

Why Crop Protection > What are Pests > Losses due to Pests > Integrated Pest Management > Integrated Pest Management Strategy >

Why Crop Protection

India with diversified agro - ecosystems responded spontaneously to the technologies of green revolution with introduction of several components in crop production like developing and adopting high yielding varieties, hybrids, usage of new agro-chemicals and adoption of intensive crop cultivation techniques.
The gains of green revolution reflected in the shape of production of 200 million tonnes of food grains, 25 million tonnes of oil seeds and 15 million tonnes of fibres per annum. But these steady gains in agricultural production over past four decades have not fully overcome the problem of rising demand caused by soaring population growth.
Adding to the population explosion, there were frequent set backs to crop production experienced in the shape of abiotic and biotic stresses during the last two decades in several food crops where intensive farm practices were adopted.
Among these stresses on major crops, increased pest populations leading to the stage of collapse of economy, at times keep the planners and executors to be helpless. In the past one and half decades, the periodical unabated explosions of aphids, whiteflies, bollworms, pod borers, defoliators, coccids, cutworms, plant hoppers etc., as direct crop damagers and disease transmitters in different regions of the country have made agriculture less remunerative and highly risk prone.
The ability of some of these pests to develop resistance curbs the effectiveness of many commercial chemicals. Resistance has accelerated in many insect species and it was reported that more than 500 insect and mite species are immune to one or more insecticides at present. Similarly about 150 plant pathogens such as fungus and bacteria are now shielded against fungicides. Some of the weedicides also found effective earlier failed to control weeds now-a-days.
Experts assessment reveal that around 22 per cent of yield losses in major crops like Rice, Cotton, Groundnut, Sugarcane, Sorghum, Tomato, Chillies, Mango, Grapes, etc., can be attributed to insect pests.
Hence, there is need to reduce if not eliminate these losses by protecting the crops from different pests through appropriate techniques. At present day the role of crop protection in agriculture is of great importance and a challenging process than before, as the so called resistant species should be brought under check.
All other management practices of crop husbandry will be futile if the crop is not protected against the ravages of pests. In absence of crop protection the yields may be drastically declined. The entire effort of growing a crop will be defeated in absence of crop protection resulting in financial loss to the grower. So the crop protection against various pests is a must in agriculture.


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What are Pests

'PEST' is an organism that causes damage resulting in economic loss to a plant or animal. It can also be said that pest is a living organism that thrives at the expense of other living organism.
The expression of "Pest" is used very broadly to insects, other invertebrates like nematodes, mites, snails and slugs, etc., and vertebrates like rats, birds, jackals, etc., that cause damage to crops, stored products and animals.
Disease producing pathogens of plants and weeds are also referred as crop pests.


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Losses caused due to pests

It is a well known fact that insects being widely distributed became more problematic in tropical climate. Of 1.5 million species of insects so far described few are so conspicuous in their presence due to their ability to develop rapidly and becoming serious by attacking food crops directly and indirectly.
In developing country like ours insects are dominating over other pests by acquiring characters like resistance to toxic chemicals, and resurgence, particularly in intensive crop management regions of the country. The losses caused by insect pests like Spodoptera, Heliothis, Whitefly and Aphids are so enormous that these made the farmer to disturb the present ecosystems with continuous use of excessive insecticides.
The losses caused by different pests and monitory losses incurred as a result of loss is furnished below

Pests
Loss caused (in percentage)
Monitory loss in crores (Rs.)
Insects
20
1200
Storage Pests
7
420
Diseases
26
1560
Weeds
33
1980
Rodents
6
360
Miscellaneous
8
480
Total
100
6000
Source : Pesticide Information April - June, 1995.


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Integrated Pest Management (Ipm)

What is Ipm ?

IPM is a system that in the context of the associated environment and the population dynamics of the pest species utilizes all suitable techniques and methods in as compatible manner as possible and maintains the pest populations at levels below those causing economic injury (FAO, 1972). In integrated pest management both crop and pest are seen as part of a dynamic agro-ecosystem.
IPM attempts to capitalize on natural biological factors that limit pest out breaks, only using chemicals as a last resort. The goal is to reduce crop damage to a level where it is economically tolerable, using control measures whose cost both economic and ecological is not excessive. A number of non-chemical cultural practices form the core of IPM. But IPM does not preclude chemical pesticide usage. Pesticide usage is one of weapons in the management armoury to us that can be exploited sensibly and judiciously.
IPM In Sustainable Agriculture

For sustainable agriculture IPM is location specific and resource oriented process in terms of ,
Preserving land races of the crops that can with stand biotic and abiotic stresses.
Restoring ecobalance to the extent possible.
Highlighting the importance of organic nutrition.
Dovetailing traditional methods with modern techniques.
Involving physical labour along with agro-machinery and chemical means.
Developing human resource to understand the recyclic (energy transfer) phenomenon of agro-ecosystems.
Leading to less risk-prone vis-a-vis low input oriented agriculture.
Acceptable socially, economically and politically.


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Integrated Pest Management Strategy

While developing IPM strategy one has to select different components that are readily available, economical and applicable at field level.
To cater the needs for location specific cropping systems the suitable technologies should be developed by Research workers from time to time.
The research findings that are practically implementable should be popularized by the Extension workers through education to farming community. Farmers have to be trained in scouting, diagnosis of pest infestation and arriving ETLs for need based chemical application in time.
Farmers should also be trained in selection of suitable pesticide, use of proper lethal dose and proper coverage of foliage to avoid risks of resistance, resurgence and residues. Farmers training is a continuous process and is an important integrated part for successful implementation of IPM.
An healthy, meaningful co-operation is very much needed from corporate pesticide industry to make IPM successful at farmers level. The pesticide industry should not wield enormous financial power and maintain market dominance against ecological and environmental safety.
When to use Crop Protection Chemicals

When adult activity is in increasing trend resulting in unacceptable pest load on crop as indicated by pheromone, light and sticky traps.
When field scouts fixed plot survey indicate a particular dominating stage of pest in the field.
When the bio-agents existing in the area did not attain a level, that can influence the pest population.
When insecticide resistance due to usage of insecticides does not surface practically.
When residues of insecticides do not become problematic.
When the role of bio-agents and other environmental resistance factors are less perceptible.
How can a Crop be monitored

A field crop is monitored to determine a pests economic status or to determine whether a natural enemy is at a level capable of suppressing a pest's population density. So identification of pests and beneficial insects is of prime importance before any control operation is executed.
Monitoring tools like pheromone, light and sticky traps can be advantageously used. Field scouting adopting fixed plot survey or roving survey should be taken from time to time to monitor the crop in determining whether the pest population attained ETLs.
Which Products Form Part of The Ipm Strategy

Different monitoring tools like pheromone traps, light traps, coloured sticky traps.
Preserved specimens of pests, natural enemies, infested plant portions as identification tools.
Bird perches.
Seed dressing chemicals and seed dressing machines.
Seeds of Resistant varieties.
Ecofriendly insecticides like Neem products and bio-fungicides like Trichoderma sp.
Natural enemies like Trichogramma egg cards, and microbial preparations of NPV & Bt.
Soft and target specific pesticides.
Bait preparations.
Good plant protection equipment.
Finally mostly farm based renewable resources that can enhance the recycling phenomenon of ecosystem should form part of IPM strategy.

TOMATO FERTILIZER

Why Fertilizers

Increasing agricultural production in India by area increasing process is no longer possible as cultivable land left over is only marginal. Further a considerable cultivable land is being diverted year after year for industrial purpose and housing etc. Hence self sufficiency in food lies in increasing the yield per unit area per unit time through adoption of modern agricultural technology.
It is universally accepted that the use of chemical fertilizers is an integral part of the package of practices for raising the agricultural production to a higher place. Studies conducted by the Food and Agricultural Organization of the United Nations (FAO) have established beyond doubt that there is a close relationship between the average crop yields and fertilizer consumption level. More-over the nutritional requirement of different crops could not be fully met with the use of organic manures like FYM and other bulky organic manures like Neem cake, Castor cake, Groundnut cake, etc., for want of their availability in adequate quantities.
Further fertilizers have the advantages of smaller bulk, easy transport, relatively quick in availability of plant-food constituents and the facility of their application in proportion suited to the actual requirements of crops and soils. Hence there is need for an efficient use of fertilizers as major plant nutrient resource in enhancing the farm productivity. Other resource of plant nutrients like organic manures, bio-fertilizers etc., also should be integrated to get the maximum agricultural output from every kilogram of applied nutrient in the form of fertilizers.





Nutrients Required By Plants

Plants require 16 essential elements for their normal growth and development.
The essential elements exist as structural components of a cell, maintain cellular organizations, function in energy transformations and in enzyme reaction.
Carbon, Hydrogen and Oxygen are three naturally occurring nutrients and form about 94 per cent of the dry weight of plants. These are the major components of carbohydrates, proteins and fats. Besides their structural role, they provide energy required for the growth and development of plants by oxidative breakdown of carbohydrates, proteins and fats during cellular respiration.
Nitrogen, Phosphorus and Potassium are three major or primary nutrients which are to be made available in larger quantities.
Nitrogen is an essential constituent of metabolically active compounds such as aminoacids, proteins, enzymes and some non-proteinous compounds. When nitrogen is a limiting factor, the rate and extent of protein synthesis are depressed and as a result plant growth is affected. The plant gets stunted and develops chlorosis.
Phosphorus is a structural component of all membranes, chloroplasts and mitochondria and a constituent of sugar phosphates, viz., ADP, ATP, nucleic acid, Phospholipids and phosphatides. Phosphorus plays an important role in energy transformations and metabolic processes in plants. It stimulates root growth.
Potassium plays an important role in the maintenance of cellular organisations by regulating permeability of cell membranes and keeping the protoplasm in a proper degree of hydration. It activates the enzymes in protein and carbohydrate metabolism and translocation of carbohydrates and imparts resistance to plants against fungal and bacterial disease.
Calcium, magnesium and sulphur are secondary nutrients which are required in relatively smaller but in appreciable quantities. Calcium, a constituent of the cell wall, an activator of different plant enzymes and is essential for the stability of cell membranes.
Magnesium is a constituent of chlorophyll and chromosome. It is known to play a catalytic role as an activator of a number of enzymes, most of w.hich are concerned with carbohydrate metabolism.
Sulphur is required to synthesize the sulphur containing amino acids and proteins, activity of proteolytic enzymes and increases oil content in oil bearing plants.
Iron, zinc, manganese, copper, boron, molybdenum and chlorine are required by plants in small quantities for their growth and development. Hence they are known as micronutrients or trace elements. The very fact that the micronutrient elements are required by plants in very low concentration suggests that they all function as catalysts or at least closely linked with some catalytic processes in plants. Manganese, zinc and copper are components of certain biological oxidation-reduction systems. Manganese performs some function in photosynthesis, acts as regulator to the intake and state of oxidation of certain elements. Zinc is concerned with the functioning of Sulphydryl compounds such as cystein, in the regulation of oxidation - reduction potential within the cells. Copper is a constituent of cytochrome oxidase and component of many enzymes like ascorbic acid oxidase, phenolase and lactase. Molybdenum is a constituent of nitrate reductase and nitrogenase enzyme and is associated with nitrogen utilization and in nitrogen fixation. Chlorine stimulates the activity of some enzymes and influences carbohydrate metabolism.
Boron helps in cell development by its influence on polysaccharide formation. It regulates translocation of sugars across membranes and polyphenolase activity. Iron is a constituent of cytochromes, haem and non haem enzymes. Perhaps the best known role of iron is its catalytic role in enzyme activity.





Diagnosis of Fertilizer Requirement

For obtaining maximum crop yields with maximum benefit to the cultivators, it is most essential that the crop plants should be fed properly with all nutrients. Soils deficient in particular nutrients must be supplied with fertilizers containing those plant nutrients.
Thus it is important to know which plant nutrients are lacking in a soil. Simple and elaborate tests have been developed by the agricultural scientist to estimate the nutritional requirements of soils and crops. These methods are known as diagnostic techniques. Fertilizer requirement is known by different diagnostic techniques and they are as follows ;
By Plant Observation

This is one of the method to know the fertilizer need of plants by means of the hunger signs of plants which can be detected by the eye.
The basis of the method is the fact that the plant suffering from severe deficiencies and excess of mineral nutrients usually developed well-defined and typical sign of disorders in various organs, particularly in the leaves. Usually, specific abnormal colours are developed in the leaves due to deficiency of plant nutrients.
Although the hunger signs in plants are easily observed, it is not easy to recognise the particular nutrient deficiency in nature due to various field conditions. This requires experience and practice in the field.
By Plant Analysis

The use of plant analysis as a tool to diagnose fertility status mainly consists of :
Plant tissue tests or rapid tests,
Total analysis,
Biochemical methods.
The basis of plant analysis for diagnostic purposes is that the amount of a given nutrient in a plant is an indication of the supply of that particular nutrient and is directly related to the quantity present in the soil. The normal growth of a plant is determined by the supply of the nutrients. However, there is one disadvantage with this method, that is, while the shortage of one nutrient can limit the growth, other nutrients may show higher contents in the cell sap irrespective of the supply.
The use of plant tissue tests as a means to diagnose soil fertility status has been found to be important. This is a rapid test of the cell sap of the growing plants. The sap from the ruptured cells is tested for unassimilated nitrogen, phosphorus, potash and other nutrients. Tissue tests are getting popular because of the convenience of handling and the small number of equipment needed for the test. The test can be made in a few minutes.
Total analysis is used extensively in research work as this gives a quantitative indication of the level of nutrients in plants. However, it should be remembered that the determination of total analysis gives both the assimilated and unassimilated nutrients. Many nutrients such as N, P, K, Ca, Mg, Mn, Zn, Cu, Fe, Mo and B can be determined by this method. Usually, the mature plants are selected for this testing.
Biochemical methods to determine the soil fertility require costly equipments, but offer good opportunities for research work. Two methods are recognised amongst biological tests. They are, use of higher plants, Microbiological methods.
By Fertilizer Experiments

In India, simple field experiments on farmers fields as well as complex field experiments are very popular.
Simple Field Experiments - In well managed state farms, the level of soil fertility is usually higher than in the farmers fields. This is due to the use of manures, fertilizers, good management practices, etc. Many experiments conducted on farmers fields have revealed the deficiency of nutrients at various levels. These experiment have to be simple in nature with N, P, K, NP, NK, PK, NPK as the treatments.
These simple field experiments on farmers fields are very educative and effective for the farmers, as they themselves see the deficiencies and the response of the nutrients. These trials are useful for advising the correct type and amount of fertilizer.
Complex Field Experiments

Complex field experiments allow the testing of many factors at a time and permit a study of interaction among various nutrients. Complex fertilizer trials helps in determining the correct kinds of fertilizer, amount and the method of application for each of the soil zone. These experiments are complicated, expensive and can be done only by experienced people.
By Soil Testing

Soil testing is one reliable diagnostic tool whose value in evaluating soil-fertility conditions has been recently recognised in India. Soil testing is multipurpose in nature. Its purposes are :
To group soils into classes relative to the levels of nutrients for suggesting fertilizer practices.
To predict the probability of getting a profitable response to the application of fertilizers.
To help evaluate soil profitability and To determine specific soil conditions i.e., alkalinity, salinity, acidity, that limit crop yields and can be improved with soil amendments and other management practices.





Organic Fertilizers and Manures

Organic fertilizers include both plant and animal bi-products. They are slow acting. Organic nitrogen fertilizers include oil cakes, fish manure, dried blood from slaughter houses etc., where as organic phosphorus from bone meal and organic potassium from cattle dung ash, wood ash, leaf mould, tobacco stems and water hyacinth.
Organic Manures

Manures are organic or inorganic substances applied to the soil to supply one or more nutrients to plants to obtain increased yields.
Manures are classified as follows
Manures

Organic manures Inorganic manures
Bulky Concentrated Artificial
Bulky (Slow acting with large quantities of organic matter) Eg: Cattle, Sheep Poultry, Pig, Goat,, Horse manures, Compost, Green Manures, Sewage.Sludge. Concentrated(Quick acting with small quantity of organic matter.Eg: Groundnut cake, Castor cake, Bonemeal, Blood meal, Horn meal, Wood ash, Cotton and Linseed Meal. (Artificial manures,Chemical fertilizers very quick acting with No organic matter.Eg: Nitrogenous, Ammonium,Phosphatic, Potassic and Sulphate fertilizers.





Inorganic Fertilizers

Nitrogen

Nitrogen is the first fertilizer element of the macronutrients usually applied in commercial fertilizers. Nitrogen is very important nutrient for plants and it seems to have the quickest and most pronounced effect.
Role of Nitrogen In Plants

Nitrogen is of special importance in the formation of protein in plants,
It forms a constituent of every living cells in the plants,
It is also present in chlorophyll,
It is involved in photosynthesis, respiration and protein synthesis,
It plays an important role in vegetative growth and it imparts dark green colour to plants.
If excess nitrogen is applied it delays ripening by encouraging more vegetative growth. The leaves acquire a dark green colour, become thick and leathery and in some cases crinkled. The plants become more liable to attack of pests and diseases. In case of cereal crops, the straw becomes weak, and the crop very often lodges and straw and grain ratio is increased. Excess nitrogen deteriorates the quality of some crops such as potato, barley and sugarcane. It delays reproductive growth and may adversely affect fruit and grain quality.
The deficiency of Nitrogen leads to formation of yellowish or light green coloured leaves and plant become stunted. The leaves and young fruits tend to drop prematurely. The kernels of cereals and the seed of other crops do not attain their normal size, and become shrivelled and light in weight.
Phosphorus

Phosphorus is the second fertilizer element and it is an essential constituent of every living cells and for the nutrition of plant and animal. It takes active part in all types of metabolism of plant. It is an essential constituent of majority of enzymes and also structural component of membrane system of cell, chloroplasts and the mitochondria. It is intimately associated with the life process.
Phosphorus stimulates root development and growth in the seedling stage and there by it helps to establish the seedlings quickly. It hastens leaf development and encourages greater growth of shoots and roots. It enhances the development of reproductive parts and thus bringing about early maturity of crops particularly the cereals. It increases the number of tillers in cereal crops and also strengthen the straw and thus helps to prevent the lodging. It stimulates the flowering, fruit setting and seed formation and the development of roots, particularly of root crops. Phosphorus has a special action on leguminous crops. It induces nodule formation and rhizobial activity.
Excess phosphorus leads to profuse root growth, particularly of the lateral and fibrous rootlets. It leads to some trace element deficiencies particularly iron and zinc.
Deficiency of phosphorus leads to restricted root and shoot growth, leaves may shed prematurely, flowering and fruiting may be delayed considerably. In case of potato tubers phosphorus deficiency leads to formation of rusty brown lessions.
Potassium

Potassium is the third fertilizer element. Potassium acts as a chemical traffic policeman, root booster, stalk strengthener, food former, sugar and starch transporter, protein builder, breathing regulator, water stretcher and as a disease retarder but it is not effective without its co-nutrients such as nitrogen and phosphorus.
Potassium is an essential element for the development of chlorophyll. It plays an important role in photosynthesis, i.e., converting carbon-dioxide and hydrogen into sugars, for translocation of sugars, and in starch formation. It improves the health and vigour of the plant, enabling it to withstand adverse climatic condition. It increases the crop resistance to certain diseases. Potash plays a key role in production of quality vegetables. Potassium is an enzyme activator and increases the plumpness and boldness of grains and seeds. It improves the water balance. Promotes metabolism and increases the production of carbohydrates.
Potassium deficiency causes stunting in growth with shortening of internodes and bushy in appearance, brings about chlorosis, i.e., yellowing of leaves and leaf scorch in case of fruit trees. It is also responsible for the 'dying back tips' of shoots. Its deficiency leads to reduction in photosynthesis, blackening of tubers in case of potato, tips or margin of lower leaves of legumes, maize, cotton, tobacco and small grains are either scorched or burnt.
Secondary Nutrients

Secondary nutrients include calcium, magnesium and sulphur, which play an important role in plant growth and development. The details of these nutrients are given below.
Calcium

Calcium as calcium pectate is an important constituent of cell wall and required for cell division. It is a structural component of chromosomes. It includes stiffness to straw and there by tends to prevent lodging. It enhances the nodule formation in legumes, helps in translocation of sugars, neutralizes organic acids which may become poisonous to plants. It is an essential co-factor or an activator of number of enzymes. It improves the intake of other plant nutrients, specially nitrogen and trace elements by correcting soil pH. Excessive amounts of calcium can decrease the availability of many micronutrients.
Deficiency of calcium lead to 'Die back' at the tips and margins of young leaves. Normal growth of plants is arrested i.e., roots may become short, stubby and bushy, leaves become wrinkled and the young leaves of cereal crops remain folded. The acidity of cell sap increases abnormally and it hampers the physiological function of plant. As a result of which plant suffers and causes the death of plant at last.
Magnesium

Magnesium is an essential constituent of chlorophyll. Several photosynthetic enzymes present in chlorophyll requires magnesium as an activator. It is usually needed by plants for formation of oils and fats. It regulates the uptake of nitrogen and phosphorus from the soil. Magnesium may increase crop resistance to drought and disease.
Deficiency of magnesium leads to yellowing of the older leaves known as chlorosis. Acute deficiency of magnesium also causes premature defoliation. In case of maize the leaves develop interveinal white strips, in cotton they change to purplish red, veins remain dark green, in soybean they turn yellowish and in apple trees, brown patches (blotches) appear on the leaves.
Sulphur

Sulphur has specified role in initiating synthesis of proteins. Sulphur is an important nutrient for oil seeds, crucifers, sugar and pulse crops. It is an essential constituent of many proteins, enzymes and certain volatile compounds such as mustard oil. It hastens root growth and stimulates seed formation. It is essential for the synthesis of certain aminoacids and oils. It can be called as master nutrient for oilseed production.
The deficiency of sulphur leads to slow growth with slender stalks, nodulation in legumes may be poor and nitrogen fixation is reduced. The young leaves turn yellow and the root and stems become abnormally long and develop woodiness. In case of fruit trees, the fruits become light green, thick skinned and less juicy. Sulphur deficient plant produces less protein and oil.
Micronutrients

Micronutrient elements are required by plants in very low concentration suggests that they all function as catalyst or atleast closely linked with some catalytic process in plants. Micronutrient elements include boron, copper, zinc, iron, manganese, molybdenum and chlorine.
Boron helps in cell development by its influence on polysaccharide formation. It regulates translocation of sugars across membranes and polyphenolase activity. Iron is a constituent of cytochrome, haem and non-haem enzymes. Perhaps the best known role of iron is its catalytic role in enzyme activity.
Copper, zinc and manganese are components of certain biological oxidation-reduction systems. Manganese performs some function in photosynthesis, acts as regulator to the intake and state of oxidation of certain elements.
Zinc is concerned with the formation of Sulphydryl compounds such as cystein in the regulation of oxidation-reduction potential within the cells. Molybdenum is a constituent of nitrate reductase and nitrogenase enzyme and is associated with nitrogen utilization and in nitrogen fixation. Chlorine stimulates the activity of some enzymes and influences carbohydrate metabolism.





Fertilizer Application

Placement

Inserting or drilling or placing the fertilizer below the soil surface by means of any tool or implement at desired depth to supply plant nutrients to crop before sowing or in the standing crop is called placement.
With placement methods, fertilizers are placed in the soil irrespective of the position of seed, seedling or growing plants before sowing or after sowing the crops. The following methods are most common in this category.
Plough - Sole Placement

In this method, the fertilizer is placed in a continuous band on the bottom of the furrow during the process of ploughing. Each band is covered as the next furrow is turned. No attempt is usually made to sow the crop in any particular location with regard to the plough sole bands.
This method has been recommended in areas where the soil becomes quite dry up to a few inches below the soil surface during the growing season, and especially with soils having a heavy clay pan a little below the plough-sole. By this method, fertilizer is placed in moist soil where it can become more available to growing plants during dry seasons.
Deep Placement of Nitrogenous Fertilizers

This method of application of nitrogenous and phosphatic fertilizers is adopted in paddy fields on a large scale in Japan and is also recommended in India. In this method, ammonical nitrogenous fertilizer like ammonium sulphate or ammonium forming nitrogenous fertilizer like urea, is placed in the reduction zone, where it remains in ammonia form and is available to the crop during the active vegetative period.
Deep or sub-surface placement of the fertilizer also ensures better distribution in the root zone and prevents any loss by surface drain-off. Deep placement is done in different ways, depending upon the local cultivation practices. In irrigated tracts, where the water supply is assured, the fertilizer is applied under the plough furrow in the dry soil before flooding the land and making it ready for transplanting. In areas where there is not too much of water in the field, it is broadcast before puddling. Puddling places the fertilizer deep into the root zone.
Sub - Soil Placement

This refers to the placement of fertilizers in the sub-soil with the help of heavy power machinery.
This method is recommended in humid and sub-humid regions where many sub-soils are strongly acidic. Due to acidic conditions the level of available plant nutrients is extremely low. Under these conditions, fertilizers, especially phosphatic and potassic are placed in the sub-soil for better root development.
Localised Placement

This method refers to the application of fertilizers into the soil close to the seed or plant.
Localised placement is usually employed when relatively small quantities of fertilizers are to be applied. Localised placement reduces fixation of phosphorus and potassium.
Bulk Blending

It is the process of mixing two or more different fertilizers varying in physical and chemical composition without any adverse effects.
For this formulation certain additional materials called 'Fillers' and 'Conditioners' are used to improve the physical condition of the mixed fertilizer. This mixed fertilizer should be applied as top dressing.
Liquid Fertilization

The use of liquid fertilizers as a means of fertilization has assumed considerable importance in foreign countries. Solutions of fertilizers, generally consisting of N, P2O5, K2O in the ratio of 1 : 2 : 1 and 1 : 1 : 2 are applied to young vegetable plants at the time of transplanting. These solutions are known as 'Starter Solutions'.
They are used in place of the watering that is usually given to help the plants to establish. Only a small amount of fertilizer is applied as a starter solution. The starter solution has two advantages.
The nutrients reach the plant roots immediately,
The solution is sufficiently diluted so that it does not inhibit growth.
As such a starter solution helps rapid establishment and quick early growth. There are two disadvantages of starter solution, if watering is not a part of the regular operation-extra labour is necessary and the fixation of phosphate may be greater.
Direct application of liquid fertilizers to the soil need special equipment. Anhydrous ammonia (a liquid under high pressure upto 14 kg per square cm. Or more) and nitrogen solutions are directly applied to the soil. This practice is very popular in the United States of America. Plant injury or wastage of ammonia is very little if the material is applied about 10 cm below the seed. If the application is shallow, nitrogen from ammonia will be lost. This method allows direct utilisation of the cheapest nitrogen source.
Straight and mixed fertilizer containing N, P and K easily soluble in water, are allowed to dissolve in the irrigation stream. The nutrients are thus carried into the soil in solution. This practice of fertilization is called "Fertigation". This saves the application cost and allows the utilization of relatively in expensive water-soluble fertilizers. Usually nitrogenous fertilizers are most commonly applied through irrigation water.
Foliar Application

This refers to the spraying on leaves of growing plants with suitable fertilizer solutions. These solutions may be prepared in a low concentration to supply any one plant nutrient or a combination of nutrients.
It has been well established that all plant nutrients are absorbed through the leaves of plants and this absorption is remarkable rapid for some nutrients. Foliar application does not result in a great saving of fertilizer but it may be preferred under the following conditions.
When visual symptoms of nutrient deficiencies observed during early stages of deficiency.
When unfavourable soil physical and chemical conditions, which reduce fertilizer use efficiency (FUE).
During drought period where in the soil application could not be done for want of soil moisture.
There are certain difficulties associated with the foliar application of nutrients as detailed below,
Marginal leaf burn or scorching may occur if strong solutions are used.
As solutions of low concentrations (usually three to six per cent) are to be used, only small quantities of nutrients can be applied in single spray.
Several applications are needed for moderate to high fertilizer rates, and hence
Foliar spraying of fertilizers is costly compared to soil application, unless combined with other spraying operations taken up for insect or disease control.





Soil Fertility and its Importance

Soil fertility may be defined as the inherent capacity of soil to supply plant nutrients in adequate amount and in suitable proportion and free from toxic substances. There are two types of soil fertility viz.,
Inherent or Natural Fertility

The soil, as a nature contain some nutrients, which is known as inherent fertility. Among plant nutrients nitrogen, phosphorus and potassium is essential for the normal growth and yield of crop. The inherent fertility has a limiting factor from which the fertility is not decreased.
Acquired Fertility

The fertility develops by application of manures and fertilizers, tillage, irrigation, etc., is known as acquired fertility.
The acquired fertility has also a limiting factor. It is found by experiment that the yield does not increase remarkably by application of additional quantity of fertilizers.
Factors Effecting Soil Fertility

The factors that are effecting soil fertility may be of two types, i.e.,
Natural factors and
Artificial factors
The natural factors are those which influences the soil formation and the artificial factors are related to the proper use of land.
The factors effecting the fertility of soil are parent material, climate and vegetation, topography, inherent capacity of soil to supply nutrient, physical condition of soil, soil age, micro-organisms, availability of plant nutrients, soil composition, organic matter, soil erosion, cropping system and favourable environment for root growth.
Maintenance of Soil Fertility

Maintenance of soil fertility is a great problem of our farmers. Cultivation of particular crop year after year in the same field decreases the soil fertility. To increase the soil fertility, it is necessary to check the loss of nutrient and to increase the nutrient content of soil.
The following things must be properly followed for increasing the fertility of soil.
Proper use of land,
Good tillage,
Crop rotation,
Control of weeds,
Maintenance of optimum moisture in the soil,
Control of soil erosion,
Cultivation of green manure crops,
Application of manures,
Cultivation of cover crops,
Removal of excess water, (drainage)
Application of fertilizers,
Maintenance of proper soil reaction.





Soil Reaction and Liming

It is well known fact that in high rainfall areas, due to the leaching of bases, acids soils are formed, while in low rainfall regions, on account of arid and semi arid conditions, saline and alkali soils occur.
Thus soil vary in acidity or alkalinity. The soil reaction is indicated by pH scale. When Ca(OH)2 or lime is added to the soil, it will become alkaline.
Liming of Acidic Soils

Liming means addition of any compound containing Calcium alone or both calcium and magnesium, that is capable of reducing the acidity of the soil. Lime correctly refers only to Calcium oxide (CaO), but the term as applied in agriculture is universally used to include various other materials also, like Calcium carbonate, Calcium hydroxide, Calcium - magnesium carbonate (marl) and Calcium silicate slags.
The effects of liming on the soil and plants are as follows :
Lime neutralizes soil acidity,
Beneficial soil bacteria are encouraged by adequate supplies of lime in the soil,
Lime makes phosphorus more available,
Liming helps the availability of potash and molybdenum,
Lime furnishes two essential elements, namely calcium and magnesium (if lime is dolamitic) for plant nutrition,
Lime reduces toxicity of Al, Mn and Fe,
Improves soil physical conditions.





Fertilizers and Environmental Pollution

Fertilizers are relatively safer than pesticides which exhibit toxic properties on living systems. However, all the quantities of fertilizers applied to the soil are not fully utilized by plants. About 50 per cent of fertilizers applied to crops are left behind as residues. Though, inorganic fertilizers are not directly toxic to man and other life forms, they have been found to upset the existing ecological balance. The nutrients escape from the fields and are found in excessive quantities in rivers, lakes and coastal waters.
Algae blooms occur when the nutrient load is high, and these smother other aquatic vegetation and also interfere with the oxygen regulation in the water bodies. This phenomena may lead to loss of fish. Among the major synthetic plant nutrients, nitrogenous fertilizers cause most harm. Contamination of the environment arises because not all the fertilizer applied is taken up by the crop and removed at harvest. In tropical climate the maximum recovery in dry land crops is 50 to 60 per cent and 40 per cent in rice because much of nitrogen is lost as ammonia into the atmosphere.
Eutrophication of water bodies due to higher nitrate and phosphate concentrations, increasing levels of nitrates in drinking water sources, accumulation of heavy metals such as lead and cadmium in soils and water resources are the principal causes of environmental concerns due to fertilizer use in agriculture. In the a national wide survey it was found that many streams and more than 20 % of wells contain 10 to 50 mg or even more of nitrates per litre of water. The contamination is caused by domestic sewage leaking to the ground water. The nitrates in drinking water can lead to several ailments. Blue - baby syndrome in infants and gastric and other forms of cancer have been related with nitrates in drinking water or diet.
Another hazard associated with excessive use of fertilizers is the gaseous loss of nitrogen, into the atmosphere. High doses of carbon dioxide and ammonia that escape into the atmosphere both from fertilizer manufacturing plants and soils affect human health. Further the oxides of nitrogen have been reported to adversely affect the ozone layer, which protects the earth from UV radiation and heating up of earth.
The oxides of nitrogen cause respiratory diseases like asthma, lung cancer and bronchitis. Arsenic, ammonia are waste stream components of nitrogen manufacturing plants while fluoride, cadmium, chromium, copper, lead and manganese are waste stream components of phosphatic fertilizer industry. If these waste stream of components are not properly disposed they cause harm to human beings and animals with contamination of air and water.
The keeping quality of perishables like vegetables and fruits get declined with excess use of fertilizers particularly nitrogenous fertilizers.





Economics of Fertilizer Use

Use of fertilizer by the farmer for increased crop production depends almost entirely on its economics. This is usually done by reporting response per unit area or per unit nutrient applied. With a view to convince the farmer about the profitability of fertilizer use, cost benefit ratio is also worked out.
Almost all such calculations are based on evaluating the extra produce at the support/market price and deducting the cost of fertilizer only at the statutory prevailing rates.
Due to high cost of commercial fertilizer marketed in India, the question of economics of fertilizer use has assumed great importance. The fertilizer association of India, New Delhi, therefore, organised series of group discussions on "Economics of Fertilizer use" during 1975. The recommendations of these group discussions are listed below,
Uniformity of approach in studying the economics of fertilizer is essential.
The fertilizer recommendations should be based on soil test values.
Balanced use of fertilizer should be advocated for better economic returns.
Use of nitrogenous fertilizer in split doses economises fertilizer use.
Micronutrient deficiencies should be corrected as and when needed.
Fertilizer schedule should be adopted for the whole crop sequence instead of a single crop.
To get the maximum benefit from the applied fertilizers, crops should be irrigated at the critical growth stages.





Nutrient Removal by Crops

Crop

Average Yield

Nutrient removed from the soil to produce an average yield(kgs/Hac)

Nutrient requirement (kgs)/ ton of produce

Recommended doses (kgs/hac)

Target yeild (t/Hac)

Nutrient requirement (kgs/hac to produce targeted yeild

Kharif

Rabi

N

P2O5

K2O

N

P2O5

K2O

Zones

N

P2O5

K2O

N

P2O5

K2O

N

P2O5

K2O

Paddy

2.24 t/hac

34

22

67

14.7

6

17.4

K-G delta

60

60

40

120

60

40

6.0

88.2

36

104.4









N.coastal

80

60

40

120

60

50













S.region

80

60

40

120

60

40













N.telangana

100

50

40

120

60

40













S.telangana

120

60

40

120

60

40













LowR.f

160

80

80

-

-

-













H.altitude

80

60

50

-

-

-





Cotton

0.74 MT/hac

30

17

45

59.4

19.1

60.9

Coastal-




-

-

-

2.5

148.5

47.75

152.25









varieties

90

45

45

-

-

-













hybrids

120

60

60

-

-

-













R.seema




-

-

-













Hybrids

120

60

60

-

-

-













Telangana




-

-

-













A.variety

90

45

45

-

-

-













Hybrids

120

60

60

-

-

-





Sugar-cane

67.2 t/hac

90

17

202

0.66

0.59

1.61

SRK,VJN, VSP,MDK

112.5

100

120

-

-

-

100

66

59

161









E.G,W.G, KRS,GNT

167.5

100

120

-

-

-













CDP,KNL, ATP,CHT

225

100

120

-

-

-













NZB- EKSALI

250

100

120

-

-

-













NZB-ADSALI

400

100

120

-

-

-





Maize

2.02 t/hac

36

20

39

27.7

6.6

14.5

Rainfed

90

50

40

-

-

-













Irrigated

120

60

50

-

-

-

6

166.2

39.6

87

Chilies

-

-

-

-

19

2.5

16

Rainfed

60

40

50

-

-

-













Irrigated

200

60

80

-

-

-

6

114

15

96






Practical Recommendations

For good tillering 'P' fertilizers
For good growth 'N' fertilizers
For quality produce 'K' fertilizers
For correcting 'KHAIRA' disease in rice 'Zn' fertilizer
For correcting yellowing in Groundnut 'Fe' fertilizer
For correcting top sickness of tobacco 'B' fertilizers
For correcting Exanthema and Dieback in citrus 'Cu' fertilizers

Saturday, January 30, 2010

BRINJAL POST HARVESTING 5

Post Harvest

Post Harvest > Infrastructure > Storage > Types of Storage > Methods of Storage > Transportation > Marketing >

Post Harvest operations

Post-harvest operations are assuming importance due to higher yields and increased cropping intensity. Due to introduction of modern technology, yield levels have substantially increased resulting in a marketable surplus which has to be stored till prices are favourable for sale. With increase in irrigation facilities and easy availability of fertilizers, intensive cropping is being practiced.
Harvesting assumes considerable importance because the crop has to be harvested as early as possible to make way for another crop. Sometimes, harvesting time may also coincide with heavy rainfall or severe cyclone and floods. In view of these situations suitable technology is, therefore, necessary for reducing the harvesting time and safe storage at farm level. The post-harvest losses are estimated to be about 25 per cent.
A recent estimate by the Ministry of Food and Civil supplies put the total preventable post-harvest losses of food grains at about 20 million tons a year, which was nearly 10 per cent of the total production. The principal adviser, planning commission stated that food grains wasted during post-harvest period could have fed up 117 million people for a year.
The important operations carried out after harvesting of the crop are threshing, drying, storage and processing.


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Infrastructure

Out of the total food grain production, more than 70 percent is with the farmer and rest is stored by governmental organizations like central warehousing corporation and Food corporation of India and traders. The godowns are the most common structures for above ground bag storage.
The godowns have all the facilities for fumigation, providing aeration and rat proof. Each of the godown can hold 5000 tonnes of bagged food grains. Grain is also stored in bulk using large silos.
For want of required storage space in godowns food grains are also stored in the open and this method of storage is known as CAP storage. Cap stands for cover and plinth. Open spaces in warehouses and elsewhere are used for storing produce. Crates are placed on floor, mats are spread on the crates and finally bags are placed over the crates.
The stacks are built in the form of domes. As protection against rain and sun the stacks are covered with thick (600 to 1000 guage) black polythene sheets and the cover is tied to the stack with the help of plastic ropes.


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Storage

Harvesting of crop is seasonal, but consumption of food grain is continuous. The market value of the produce is generally low at harvesting time. So the grower need storage facility to hold a portion of produce to meet the feed and seed requirements in addition of selling surplus produce when the marketing price is favourable.
Traders and Co-operatives at market centres need storage structures to hold grains when the transport facility is inadequate.
The government also needs storage structures to maintain buffer reserves to offset the effects produced by the vagaries of nature. Hence, there is necessity to store the produce for different periods primarily for commercial reasons. The growers, processors, transporters and warehouse men have to develop storage facilities for proper storage of food grains, oilseeds, commercial crops like Chillies, vegetables and fruits etc., and seeds intended for sowing in the following seasons.
An ideal storage facility should satisfy the following requirements

It should provide maximum possible protection from ground moisture, rains, insect pests, moulds, rodents, birds, fire, etc.,
It should provide the necessary facility for inspection, disinfection, loading, unloading, cleaning and reconditioning.
It should protect grain from excessive moisture and temperature favourable to both insect and mould development.
It should be economical and suitable for a particular situation.


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Types of Storage

Holding grain in bulk in underground is an age old method of rural storage. Wheat, Paddy, Sorghum, Fingermillet, etc., can be stored underground for a period of 2 years. These structures are simple underground dig-outs upto a depth of 5 m varying in sizes to hold from a small quantity upto 50 tonnes.
The pits are lined with brick or concrete so that moisture from walls and bottom does not damage the grain. At the time of filling a layer of straw is placed on all sides.
After the pit is filled, straw is spread over the grain and then topped with a layer of soil. Insect infestation is less in the under ground storage and it is cheaper over above ground storage structures.
This underground structure is not suitable for high rainfall and high water-table areas. Further the grain stored underground have poor appearance and musty smell.
Several types of above ground storage structures mentioned below are also in use in our country,

Mud Bins

The mud bins are made of unburnt clay mixed with straw with 1 to 3 inch thick wall and are oval, rectangular or circular. A small hole is provided at the base for taking out the grain and a larger hole is provided at the top for filling it with grain. Both the inlet and outlet holes are plugged while grain is stored.
Straw Bins

For storing paddy in humid zones dried plants are used for making temporary structures, which after being filled with grain are further reinforced from outside by winding paddy straw ropes around the whole structure. Each structure holds 2 to 6 quintals of grain.
Bukhari Bins

This is a cylindrical structure and is made of mud and split bamboo's. The bin is always placed on a wooden or a massonary plat form to prevent its contact with the ground. The capacity may vary from 3 to 10 tonnes.
Kothar Type Bins

These bins are very much similar to a timber box placed on a raised plat form, which is generally supported on pillars. Both the floor and walls are made of wooden planks, where the tiled or thatched roof is placed over it as a protection against sun and rains. The capacity may vary from 9 to 35 tonnes.
Metal Bins

Bins made of steel, alluminium R.C.C are used for storage of grains outside the house. These bins are fire and moisture proof. The bins have long durability and produced on commercial scale. The capacity ranges from 1 to 10 tonnes. Silos are huge bins made with either steel, alluminium or concrete. Usually steel and alluminium bins are circular in shape. The capacity of silo ranges from 500 to 4000 tonnes. A silo has facilities for loading and unloading grains.
The storage structures in rural areas are not ideal from scientific-storage point of view, as substantial losses occur during storage of grain from insect pests, moulds, rodents, etc. ; keeping the requirements of the farmers in view the Indian grain storage institute (IGSI), Hapur with its branch at Ludhiana and Hyderabad have developed several metal bins of different capacities for scientific storage of grain in rural areas.


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Methods of Storage

The grains are stored at three different levels, viz., at the producer's level (rural storage) trader's level and urban organizational storage. The urban organization uses modern facilities and structures like silos, warehouses and also undertaken periodical inspection, processing and treatment of grains for ensuring their quality during storage.
Generally, there are two ways of storing grains i.e.
Storage in bags and Loose or bulk storage.
In the tropical regions, the grain is stored in bags. Storage in bags requires considerable labour, but the minimum investment is enough on permanent structures and equipment. The storage in bags has the advantage of being short-term storage. Bag storage can be done under a roof of Galvanized Iron sheets, a plastic covering where grain is intended for very early onward movement. Usually no control measures against insects is needed for short-term storage. If bag storage produce is intended for long time, the control measures have to be taken against insect pests.
The bulk storage has an advantage of greater storage capacity per unit volume of space. Less labour is involved in loading and unloading and there is no need of investment in purchasing gunny bags. In bulk storage the insect infestation is also lower over bag storage. The grain can be kept for several years in bulk storage.


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Transportation

When once the grain is threshed and dried it will be transported from the field to store houses by bullock carts, or tractors by the growers. Sometimes if the market price is favourable the produce is disposed to the traders soon after drying.
The disposal of the produce, either at the village or at the market yard is, however often closely connected with financial needs of the growers and sometimes indebtedness. The traders on purchasing, transport the produce to go-down, or shops for sale to the consumers.
This transport mainly uses trucks i.e., lorries. Government agencies like Food Corporation of India etc., transport the produce from one place to another place either by road or rail (waggons) for long term storage and sometimes to export to other countries by sea (cargo). If the produce is not properly bagged and handled there will be some loss during transport.


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Marketing

In general most of the producers sell the grains at their door steps in villages, to avoid transport. At village level defective measures and weights are used by traders and also the prices paid to farmers are much lower than regulated market rates. Now-a-days farmers are encouraged to sell their produce in near by regulated markets, though some labour is involved in transport.
In regulated markets some amenities are provided for sellers and the growers can secure maximum value for their produce. In market yards several methods like cover system, open system and auction system are adopted depending on the type of produce sold. Since the rural banking system is improved the farmers to a large extent they are out of clutches of greedy private money lenders who exert pressure to dispose produce for lower price.
At present in some places the cold storage facilities are also available. Farmers can utilize these cold storage facilities for stocking their produce on payment of rent and the produce can be disposed when there is remunerative price in the market.
Though several measures are taken by government the marketing of agricultural produce is facing problems and growers are not getting the reasonable price for their produce. If production exceeds demand, price declines until the market is cleared. Prices raise when production fell short. Responses to lower or higher prices occur in the next production cycle.
Therefore, the acreage for a particular crop based on demand and the supporting prices for each commodity need to be monitored by the rulers based on demand and supply studies. The government has to bring buyers and sellers together, develop price information systems, establish consistent grades and product quality standards for better marketing of agricultural produce at all times.

BRINJAL PESTS 4

Pests

Why Crop Protection > What are Pests > Losses due to Pests > Integrated Pest Management > Integrated Pest Management Strategy >

Why Crop Protection

India with diversified agro - ecosystems responded spontaneously to the technologies of green revolution with introduction of several components in crop production like developing and adopting high yielding varieties, hybrids, usage of new agro-chemicals and adoption of intensive crop cultivation techniques.
The gains of green revolution reflected in the shape of production of 200 million tonnes of food grains, 25 million tonnes of oil seeds and 15 million tonnes of fibres per annum. But these steady gains in agricultural production over past four decades have not fully overcome the problem of rising demand caused by soaring population growth.
Adding to the population explosion, there were frequent set backs to crop production experienced in the shape of abiotic and biotic stresses during the last two decades in several food crops where intensive farm practices were adopted.
Among these stresses on major crops, increased pest populations leading to the stage of collapse of economy, at times keep the planners and executors to be helpless. In the past one and half decades, the periodical unabated explosions of aphids, whiteflies, bollworms, pod borers, defoliators, coccids, cutworms, plant hoppers etc., as direct crop damagers and disease transmitters in different regions of the country have made agriculture less remunerative and highly risk prone.
The ability of some of these pests to develop resistance curbs the effectiveness of many commercial chemicals. Resistance has accelerated in many insect species and it was reported that more than 500 insect and mite species are immune to one or more insecticides at present. Similarly about 150 plant pathogens such as fungus and bacteria are now shielded against fungicides. Some of the weedicides also found effective earlier failed to control weeds now-a-days.
Experts assessment reveal that around 22 per cent of yield losses in major crops like Rice, Cotton, Groundnut, Sugarcane, Sorghum, Tomato, Chillies, Mango, Grapes, etc., can be attributed to insect pests.
Hence, there is need to reduce if not eliminate these losses by protecting the crops from different pests through appropriate techniques. At present day the role of crop protection in agriculture is of great importance and a challenging process than before, as the so called resistant species should be brought under check.
All other management practices of crop husbandry will be futile if the crop is not protected against the ravages of pests. In absence of crop protection the yields may be drastically declined. The entire effort of growing a crop will be defeated in absence of crop protection resulting in financial loss to the grower. So the crop protection against various pests is a must in agriculture.


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What are Pests

'PEST' is an organism that causes damage resulting in economic loss to a plant or animal. It can also be said that pest is a living organism that thrives at the expense of other living organism.
The expression of "Pest" is used very broadly to insects, other invertebrates like nematodes, mites, snails and slugs, etc., and vertebrates like rats, birds, jackals, etc., that cause damage to crops, stored products and animals.
Disease producing pathogens of plants and weeds are also referred as crop pests.


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Losses caused due to pests

It is a well known fact that insects being widely distributed became more problematic in tropical climate. Of 1.5 million species of insects so far described few are so conspicuous in their presence due to their ability to develop rapidly and becoming serious by attacking food crops directly and indirectly.
In developing country like ours insects are dominating over other pests by acquiring characters like resistance to toxic chemicals, and resurgence, particularly in intensive crop management regions of the country. The losses caused by insect pests like Spodoptera, Heliothis, Whitefly and Aphids are so enormous that these made the farmer to disturb the present ecosystems with continuous use of excessive insecticides.
The losses caused by different pests and monitory losses incurred as a result of loss is furnished below

Pests
Loss caused (in percentage)
Monitory loss in crores (Rs.)
Insects
20
1200
Storage Pests
7
420
Diseases
26
1560
Weeds
33
1980
Rodents
6
360
Miscellaneous
8
480
Total
100
6000
Source : Pesticide Information April - June, 1995.


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Integrated Pest Management (Ipm)

What is Ipm ?

IPM is a system that in the context of the associated environment and the population dynamics of the pest species utilizes all suitable techniques and methods in as compatible manner as possible and maintains the pest populations at levels below those causing economic injury (FAO, 1972). In integrated pest management both crop and pest are seen as part of a dynamic agro-ecosystem.
IPM attempts to capitalize on natural biological factors that limit pest out breaks, only using chemicals as a last resort. The goal is to reduce crop damage to a level where it is economically tolerable, using control measures whose cost both economic and ecological is not excessive. A number of non-chemical cultural practices form the core of IPM. But IPM does not preclude chemical pesticide usage. Pesticide usage is one of weapons in the management armoury to us that can be exploited sensibly and judiciously.
IPM In Sustainable Agriculture

For sustainable agriculture IPM is location specific and resource oriented process in terms of ,
Preserving land races of the crops that can with stand biotic and abiotic stresses.
Restoring ecobalance to the extent possible.
Highlighting the importance of organic nutrition.
Dovetailing traditional methods with modern techniques.
Involving physical labour along with agro-machinery and chemical means.
Developing human resource to understand the recyclic (energy transfer) phenomenon of agro-ecosystems.
Leading to less risk-prone vis-a-vis low input oriented agriculture.
Acceptable socially, economically and politically.


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Integrated Pest Management Strategy

While developing IPM strategy one has to select different components that are readily available, economical and applicable at field level.
To cater the needs for location specific cropping systems the suitable technologies should be developed by Research workers from time to time.
The research findings that are practically implementable should be popularized by the Extension workers through education to farming community. Farmers have to be trained in scouting, diagnosis of pest infestation and arriving ETLs for need based chemical application in time.
Farmers should also be trained in selection of suitable pesticide, use of proper lethal dose and proper coverage of foliage to avoid risks of resistance, resurgence and residues. Farmers training is a continuous process and is an important integrated part for successful implementation of IPM.
An healthy, meaningful co-operation is very much needed from corporate pesticide industry to make IPM successful at farmers level. The pesticide industry should not wield enormous financial power and maintain market dominance against ecological and environmental safety.
When to use Crop Protection Chemicals

When adult activity is in increasing trend resulting in unacceptable pest load on crop as indicated by pheromone, light and sticky traps.
When field scouts fixed plot survey indicate a particular dominating stage of pest in the field.
When the bio-agents existing in the area did not attain a level, that can influence the pest population.
When insecticide resistance due to usage of insecticides does not surface practically.
When residues of insecticides do not become problematic.
When the role of bio-agents and other environmental resistance factors are less perceptible.
How can a Crop be monitored

A field crop is monitored to determine a pests economic status or to determine whether a natural enemy is at a level capable of suppressing a pest's population density. So identification of pests and beneficial insects is of prime importance before any control operation is executed.
Monitoring tools like pheromone, light and sticky traps can be advantageously used. Field scouting adopting fixed plot survey or roving survey should be taken from time to time to monitor the crop in determining whether the pest population attained ETLs.
Which Products Form Part of The Ipm Strategy

Different monitoring tools like pheromone traps, light traps, coloured sticky traps.
Preserved specimens of pests, natural enemies, infested plant portions as identification tools.
Bird perches.
Seed dressing chemicals and seed dressing machines.
Seeds of Resistant varieties.
Ecofriendly insecticides like Neem products and bio-fungicides like Trichoderma sp.
Natural enemies like Trichogramma egg cards, and microbial preparations of NPV & Bt.
Soft and target specific pesticides.
Bait preparations.
Good plant protection equipment.
Finally mostly farm based renewable resources that can enhance the recycling phenomenon of ecosystem should form part of IPM strategy.

BRINJAL CULTIVATION FERTILIZER 3

Nutrients

Why Fertilizers > Nutrients Required by Plant > Diagnosis of Fertilizer Requirement > Organic Fertilizers and Manures > Inorganic Fertilizers > Fertilizer Application > Soil Fertility and its Importance > Soil Reaction and Liming > Fertilizers and Environmental Pollution > Economics of Fertilizer Use > Nutrient Removal by crops > Practical Recommendations >

Why Fertilizers

Increasing agricultural production in India by area increasing process is no longer possible as cultivable land left over is only marginal. Further a considerable cultivable land is being diverted year after year for industrial purpose and housing etc. Hence self sufficiency in food lies in increasing the yield per unit area per unit time through adoption of modern agricultural technology.
It is universally accepted that the use of chemical fertilizers is an integral part of the package of practices for raising the agricultural production to a higher place. Studies conducted by the Food and Agricultural Organization of the United Nations (FAO) have established beyond doubt that there is a close relationship between the average crop yields and fertilizer consumption level. More-over the nutritional requirement of different crops could not be fully met with the use of organic manures like FYM and other bulky organic manures like Neem cake, Castor cake, Groundnut cake, etc., for want of their availability in adequate quantities.
Further fertilizers have the advantages of smaller bulk, easy transport, relatively quick in availability of plant-food constituents and the facility of their application in proportion suited to the actual requirements of crops and soils. Hence there is need for an efficient use of fertilizers as major plant nutrient resource in enhancing the farm productivity. Other resource of plant nutrients like organic manures, bio-fertilizers etc., also should be integrated to get the maximum agricultural output from every kilogram of applied nutrient in the form of fertilizers.


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Nutrients Required By Plants

Plants require 16 essential elements for their normal growth and development.
The essential elements exist as structural components of a cell, maintain cellular organizations, function in energy transformations and in enzyme reaction.
Carbon, Hydrogen and Oxygen are three naturally occurring nutrients and form about 94 per cent of the dry weight of plants. These are the major components of carbohydrates, proteins and fats. Besides their structural role, they provide energy required for the growth and development of plants by oxidative breakdown of carbohydrates, proteins and fats during cellular respiration.
Nitrogen, Phosphorus and Potassium are three major or primary nutrients which are to be made available in larger quantities.
Nitrogen is an essential constituent of metabolically active compounds such as aminoacids, proteins, enzymes and some non-proteinous compounds. When nitrogen is a limiting factor, the rate and extent of protein synthesis are depressed and as a result plant growth is affected. The plant gets stunted and develops chlorosis.
Phosphorus is a structural component of all membranes, chloroplasts and mitochondria and a constituent of sugar phosphates, viz., ADP, ATP, nucleic acid, Phospholipids and phosphatides. Phosphorus plays an important role in energy transformations and metabolic processes in plants. It stimulates root growth.
Potassium plays an important role in the maintenance of cellular organisations by regulating permeability of cell membranes and keeping the protoplasm in a proper degree of hydration. It activates the enzymes in protein and carbohydrate metabolism and translocation of carbohydrates and imparts resistance to plants against fungal and bacterial disease.
Calcium, magnesium and sulphur are secondary nutrients which are required in relatively smaller but in appreciable quantities. Calcium, a constituent of the cell wall, an activator of different plant enzymes and is essential for the stability of cell membranes.
Magnesium is a constituent of chlorophyll and chromosome. It is known to play a catalytic role as an activator of a number of enzymes, most of w.hich are concerned with carbohydrate metabolism.
Sulphur is required to synthesize the sulphur containing amino acids and proteins, activity of proteolytic enzymes and increases oil content in oil bearing plants.
Iron, zinc, manganese, copper, boron, molybdenum and chlorine are required by plants in small quantities for their growth and development. Hence they are known as micronutrients or trace elements. The very fact that the micronutrient elements are required by plants in very low concentration suggests that they all function as catalysts or at least closely linked with some catalytic processes in plants. Manganese, zinc and copper are components of certain biological oxidation-reduction systems. Manganese performs some function in photosynthesis, acts as regulator to the intake and state of oxidation of certain elements. Zinc is concerned with the functioning of Sulphydryl compounds such as cystein, in the regulation of oxidation - reduction potential within the cells. Copper is a constituent of cytochrome oxidase and component of many enzymes like ascorbic acid oxidase, phenolase and lactase. Molybdenum is a constituent of nitrate reductase and nitrogenase enzyme and is associated with nitrogen utilization and in nitrogen fixation. Chlorine stimulates the activity of some enzymes and influences carbohydrate metabolism.
Boron helps in cell development by its influence on polysaccharide formation. It regulates translocation of sugars across membranes and polyphenolase activity. Iron is a constituent of cytochromes, haem and non haem enzymes. Perhaps the best known role of iron is its catalytic role in enzyme activity.


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Diagnosis of Fertilizer Requirement

For obtaining maximum crop yields with maximum benefit to the cultivators, it is most essential that the crop plants should be fed properly with all nutrients. Soils deficient in particular nutrients must be supplied with fertilizers containing those plant nutrients.
Thus it is important to know which plant nutrients are lacking in a soil. Simple and elaborate tests have been developed by the agricultural scientist to estimate the nutritional requirements of soils and crops. These methods are known as diagnostic techniques. Fertilizer requirement is known by different diagnostic techniques and they are as follows ;
By Plant Observation

This is one of the method to know the fertilizer need of plants by means of the hunger signs of plants which can be detected by the eye.
The basis of the method is the fact that the plant suffering from severe deficiencies and excess of mineral nutrients usually developed well-defined and typical sign of disorders in various organs, particularly in the leaves. Usually, specific abnormal colours are developed in the leaves due to deficiency of plant nutrients.
Although the hunger signs in plants are easily observed, it is not easy to recognise the particular nutrient deficiency in nature due to various field conditions. This requires experience and practice in the field.
By Plant Analysis

The use of plant analysis as a tool to diagnose fertility status mainly consists of :
Plant tissue tests or rapid tests,
Total analysis,
Biochemical methods.
The basis of plant analysis for diagnostic purposes is that the amount of a given nutrient in a plant is an indication of the supply of that particular nutrient and is directly related to the quantity present in the soil. The normal growth of a plant is determined by the supply of the nutrients. However, there is one disadvantage with this method, that is, while the shortage of one nutrient can limit the growth, other nutrients may show higher contents in the cell sap irrespective of the supply.
The use of plant tissue tests as a means to diagnose soil fertility status has been found to be important. This is a rapid test of the cell sap of the growing plants. The sap from the ruptured cells is tested for unassimilated nitrogen, phosphorus, potash and other nutrients. Tissue tests are getting popular because of the convenience of handling and the small number of equipment needed for the test. The test can be made in a few minutes.
Total analysis is used extensively in research work as this gives a quantitative indication of the level of nutrients in plants. However, it should be remembered that the determination of total analysis gives both the assimilated and unassimilated nutrients. Many nutrients such as N, P, K, Ca, Mg, Mn, Zn, Cu, Fe, Mo and B can be determined by this method. Usually, the mature plants are selected for this testing.
Biochemical methods to determine the soil fertility require costly equipments, but offer good opportunities for research work. Two methods are recognised amongst biological tests. They are, use of higher plants, Microbiological methods.
By Fertilizer Experiments

In India, simple field experiments on farmers fields as well as complex field experiments are very popular.
Simple Field Experiments - In well managed state farms, the level of soil fertility is usually higher than in the farmers fields. This is due to the use of manures, fertilizers, good management practices, etc. Many experiments conducted on farmers fields have revealed the deficiency of nutrients at various levels. These experiment have to be simple in nature with N, P, K, NP, NK, PK, NPK as the treatments.
These simple field experiments on farmers fields are very educative and effective for the farmers, as they themselves see the deficiencies and the response of the nutrients. These trials are useful for advising the correct type and amount of fertilizer.
Complex Field Experiments

Complex field experiments allow the testing of many factors at a time and permit a study of interaction among various nutrients. Complex fertilizer trials helps in determining the correct kinds of fertilizer, amount and the method of application for each of the soil zone. These experiments are complicated, expensive and can be done only by experienced people.
By Soil Testing

Soil testing is one reliable diagnostic tool whose value in evaluating soil-fertility conditions has been recently recognised in India. Soil testing is multipurpose in nature. Its purposes are :
To group soils into classes relative to the levels of nutrients for suggesting fertilizer practices.
To predict the probability of getting a profitable response to the application of fertilizers.
To help evaluate soil profitability and To determine specific soil conditions i.e., alkalinity, salinity, acidity, that limit crop yields and can be improved with soil amendments and other management practices.


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Organic Fertilizers and Manures

Organic fertilizers include both plant and animal bi-products. They are slow acting. Organic nitrogen fertilizers include oil cakes, fish manure, dried blood from slaughter houses etc., where as organic phosphorus from bone meal and organic potassium from cattle dung ash, wood ash, leaf mould, tobacco stems and water hyacinth.
Organic Manures

Manures are organic or inorganic substances applied to the soil to supply one or more nutrients to plants to obtain increased yields.
Manures are classified as follows
Manures

Organic manures Inorganic manures
Bulky Concentrated Artificial
Bulky (Slow acting with large quantities of organic matter) Eg: Cattle, Sheep Poultry, Pig, Goat,, Horse manures, Compost, Green Manures, Sewage.Sludge. Concentrated(Quick acting with small quantity of organic matter.Eg: Groundnut cake, Castor cake, Bonemeal, Blood meal, Horn meal, Wood ash, Cotton and Linseed Meal. (Artificial manures,Chemical fertilizers very quick acting with No organic matter.Eg: Nitrogenous, Ammonium,Phosphatic, Potassic and Sulphate fertilizers.


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Inorganic Fertilizers

Nitrogen

Nitrogen is the first fertilizer element of the macronutrients usually applied in commercial fertilizers. Nitrogen is very important nutrient for plants and it seems to have the quickest and most pronounced effect.
Role of Nitrogen In Plants

Nitrogen is of special importance in the formation of protein in plants,
It forms a constituent of every living cells in the plants,
It is also present in chlorophyll,
It is involved in photosynthesis, respiration and protein synthesis,
It plays an important role in vegetative growth and it imparts dark green colour to plants.
If excess nitrogen is applied it delays ripening by encouraging more vegetative growth. The leaves acquire a dark green colour, become thick and leathery and in some cases crinkled. The plants become more liable to attack of pests and diseases. In case of cereal crops, the straw becomes weak, and the crop very often lodges and straw and grain ratio is increased. Excess nitrogen deteriorates the quality of some crops such as potato, barley and sugarcane. It delays reproductive growth and may adversely affect fruit and grain quality.
The deficiency of Nitrogen leads to formation of yellowish or light green coloured leaves and plant become stunted. The leaves and young fruits tend to drop prematurely. The kernels of cereals and the seed of other crops do not attain their normal size, and become shrivelled and light in weight.
Phosphorus

Phosphorus is the second fertilizer element and it is an essential constituent of every living cells and for the nutrition of plant and animal. It takes active part in all types of metabolism of plant. It is an essential constituent of majority of enzymes and also structural component of membrane system of cell, chloroplasts and the mitochondria. It is intimately associated with the life process.
Phosphorus stimulates root development and growth in the seedling stage and there by it helps to establish the seedlings quickly. It hastens leaf development and encourages greater growth of shoots and roots. It enhances the development of reproductive parts and thus bringing about early maturity of crops particularly the cereals. It increases the number of tillers in cereal crops and also strengthen the straw and thus helps to prevent the lodging. It stimulates the flowering, fruit setting and seed formation and the development of roots, particularly of root crops. Phosphorus has a special action on leguminous crops. It induces nodule formation and rhizobial activity.
Excess phosphorus leads to profuse root growth, particularly of the lateral and fibrous rootlets. It leads to some trace element deficiencies particularly iron and zinc.
Deficiency of phosphorus leads to restricted root and shoot growth, leaves may shed prematurely, flowering and fruiting may be delayed considerably. In case of potato tubers phosphorus deficiency leads to formation of rusty brown lessions.
Potassium

Potassium is the third fertilizer element. Potassium acts as a chemical traffic policeman, root booster, stalk strengthener, food former, sugar and starch transporter, protein builder, breathing regulator, water stretcher and as a disease retarder but it is not effective without its co-nutrients such as nitrogen and phosphorus.
Potassium is an essential element for the development of chlorophyll. It plays an important role in photosynthesis, i.e., converting carbon-dioxide and hydrogen into sugars, for translocation of sugars, and in starch formation. It improves the health and vigour of the plant, enabling it to withstand adverse climatic condition. It increases the crop resistance to certain diseases. Potash plays a key role in production of quality vegetables. Potassium is an enzyme activator and increases the plumpness and boldness of grains and seeds. It improves the water balance. Promotes metabolism and increases the production of carbohydrates.
Potassium deficiency causes stunting in growth with shortening of internodes and bushy in appearance, brings about chlorosis, i.e., yellowing of leaves and leaf scorch in case of fruit trees. It is also responsible for the 'dying back tips' of shoots. Its deficiency leads to reduction in photosynthesis, blackening of tubers in case of potato, tips or margin of lower leaves of legumes, maize, cotton, tobacco and small grains are either scorched or burnt.
Secondary Nutrients

Secondary nutrients include calcium, magnesium and sulphur, which play an important role in plant growth and development. The details of these nutrients are given below.
Calcium

Calcium as calcium pectate is an important constituent of cell wall and required for cell division. It is a structural component of chromosomes. It includes stiffness to straw and there by tends to prevent lodging. It enhances the nodule formation in legumes, helps in translocation of sugars, neutralizes organic acids which may become poisonous to plants. It is an essential co-factor or an activator of number of enzymes. It improves the intake of other plant nutrients, specially nitrogen and trace elements by correcting soil pH. Excessive amounts of calcium can decrease the availability of many micronutrients.
Deficiency of calcium lead to 'Die back' at the tips and margins of young leaves. Normal growth of plants is arrested i.e., roots may become short, stubby and bushy, leaves become wrinkled and the young leaves of cereal crops remain folded. The acidity of cell sap increases abnormally and it hampers the physiological function of plant. As a result of which plant suffers and causes the death of plant at last.
Magnesium

Magnesium is an essential constituent of chlorophyll. Several photosynthetic enzymes present in chlorophyll requires magnesium as an activator. It is usually needed by plants for formation of oils and fats. It regulates the uptake of nitrogen and phosphorus from the soil. Magnesium may increase crop resistance to drought and disease.
Deficiency of magnesium leads to yellowing of the older leaves known as chlorosis. Acute deficiency of magnesium also causes premature defoliation. In case of maize the leaves develop interveinal white strips, in cotton they change to purplish red, veins remain dark green, in soybean they turn yellowish and in apple trees, brown patches (blotches) appear on the leaves.
Sulphur

Sulphur has specified role in initiating synthesis of proteins. Sulphur is an important nutrient for oil seeds, crucifers, sugar and pulse crops. It is an essential constituent of many proteins, enzymes and certain volatile compounds such as mustard oil. It hastens root growth and stimulates seed formation. It is essential for the synthesis of certain aminoacids and oils. It can be called as master nutrient for oilseed production.
The deficiency of sulphur leads to slow growth with slender stalks, nodulation in legumes may be poor and nitrogen fixation is reduced. The young leaves turn yellow and the root and stems become abnormally long and develop woodiness. In case of fruit trees, the fruits become light green, thick skinned and less juicy. Sulphur deficient plant produces less protein and oil.
Micronutrients

Micronutrient elements are required by plants in very low concentration suggests that they all function as catalyst or atleast closely linked with some catalytic process in plants. Micronutrient elements include boron, copper, zinc, iron, manganese, molybdenum and chlorine.
Boron helps in cell development by its influence on polysaccharide formation. It regulates translocation of sugars across membranes and polyphenolase activity. Iron is a constituent of cytochrome, haem and non-haem enzymes. Perhaps the best known role of iron is its catalytic role in enzyme activity.
Copper, zinc and manganese are components of certain biological oxidation-reduction systems. Manganese performs some function in photosynthesis, acts as regulator to the intake and state of oxidation of certain elements.
Zinc is concerned with the formation of Sulphydryl compounds such as cystein in the regulation of oxidation-reduction potential within the cells. Molybdenum is a constituent of nitrate reductase and nitrogenase enzyme and is associated with nitrogen utilization and in nitrogen fixation. Chlorine stimulates the activity of some enzymes and influences carbohydrate metabolism.


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Fertilizer Application

Placement

Inserting or drilling or placing the fertilizer below the soil surface by means of any tool or implement at desired depth to supply plant nutrients to crop before sowing or in the standing crop is called placement.
With placement methods, fertilizers are placed in the soil irrespective of the position of seed, seedling or growing plants before sowing or after sowing the crops. The following methods are most common in this category.
Plough - Sole Placement

In this method, the fertilizer is placed in a continuous band on the bottom of the furrow during the process of ploughing. Each band is covered as the next furrow is turned. No attempt is usually made to sow the crop in any particular location with regard to the plough sole bands.
This method has been recommended in areas where the soil becomes quite dry up to a few inches below the soil surface during the growing season, and especially with soils having a heavy clay pan a little below the plough-sole. By this method, fertilizer is placed in moist soil where it can become more available to growing plants during dry seasons.
Deep Placement of Nitrogenous Fertilizers

This method of application of nitrogenous and phosphatic fertilizers is adopted in paddy fields on a large scale in Japan and is also recommended in India. In this method, ammonical nitrogenous fertilizer like ammonium sulphate or ammonium forming nitrogenous fertilizer like urea, is placed in the reduction zone, where it remains in ammonia form and is available to the crop during the active vegetative period.
Deep or sub-surface placement of the fertilizer also ensures better distribution in the root zone and prevents any loss by surface drain-off. Deep placement is done in different ways, depending upon the local cultivation practices. In irrigated tracts, where the water supply is assured, the fertilizer is applied under the plough furrow in the dry soil before flooding the land and making it ready for transplanting. In areas where there is not too much of water in the field, it is broadcast before puddling. Puddling places the fertilizer deep into the root zone.
Sub - Soil Placement

This refers to the placement of fertilizers in the sub-soil with the help of heavy power machinery.
This method is recommended in humid and sub-humid regions where many sub-soils are strongly acidic. Due to acidic conditions the level of available plant nutrients is extremely low. Under these conditions, fertilizers, especially phosphatic and potassic are placed in the sub-soil for better root development.
Localised Placement

This method refers to the application of fertilizers into the soil close to the seed or plant.
Localised placement is usually employed when relatively small quantities of fertilizers are to be applied. Localised placement reduces fixation of phosphorus and potassium.
Bulk Blending

It is the process of mixing two or more different fertilizers varying in physical and chemical composition without any adverse effects.
For this formulation certain additional materials called 'Fillers' and 'Conditioners' are used to improve the physical condition of the mixed fertilizer. This mixed fertilizer should be applied as top dressing.
Liquid Fertilization

The use of liquid fertilizers as a means of fertilization has assumed considerable importance in foreign countries. Solutions of fertilizers, generally consisting of N, P2O5, K2O in the ratio of 1 : 2 : 1 and 1 : 1 : 2 are applied to young vegetable plants at the time of transplanting. These solutions are known as 'Starter Solutions'.
They are used in place of the watering that is usually given to help the plants to establish. Only a small amount of fertilizer is applied as a starter solution. The starter solution has two advantages.
The nutrients reach the plant roots immediately,
The solution is sufficiently diluted so that it does not inhibit growth.
As such a starter solution helps rapid establishment and quick early growth. There are two disadvantages of starter solution, if watering is not a part of the regular operation-extra labour is necessary and the fixation of phosphate may be greater.
Direct application of liquid fertilizers to the soil need special equipment. Anhydrous ammonia (a liquid under high pressure upto 14 kg per square cm. Or more) and nitrogen solutions are directly applied to the soil. This practice is very popular in the United States of America. Plant injury or wastage of ammonia is very little if the material is applied about 10 cm below the seed. If the application is shallow, nitrogen from ammonia will be lost. This method allows direct utilisation of the cheapest nitrogen source.
Straight and mixed fertilizer containing N, P and K easily soluble in water, are allowed to dissolve in the irrigation stream. The nutrients are thus carried into the soil in solution. This practice of fertilization is called "Fertigation". This saves the application cost and allows the utilization of relatively in expensive water-soluble fertilizers. Usually nitrogenous fertilizers are most commonly applied through irrigation water.
Foliar Application

This refers to the spraying on leaves of growing plants with suitable fertilizer solutions. These solutions may be prepared in a low concentration to supply any one plant nutrient or a combination of nutrients.
It has been well established that all plant nutrients are absorbed through the leaves of plants and this absorption is remarkable rapid for some nutrients. Foliar application does not result in a great saving of fertilizer but it may be preferred under the following conditions.
When visual symptoms of nutrient deficiencies observed during early stages of deficiency.
When unfavourable soil physical and chemical conditions, which reduce fertilizer use efficiency (FUE).
During drought period where in the soil application could not be done for want of soil moisture.
There are certain difficulties associated with the foliar application of nutrients as detailed below,
Marginal leaf burn or scorching may occur if strong solutions are used.
As solutions of low concentrations (usually three to six per cent) are to be used, only small quantities of nutrients can be applied in single spray.
Several applications are needed for moderate to high fertilizer rates, and hence
Foliar spraying of fertilizers is costly compared to soil application, unless combined with other spraying operations taken up for insect or disease control.


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Soil Fertility and its Importance

Soil fertility may be defined as the inherent capacity of soil to supply plant nutrients in adequate amount and in suitable proportion and free from toxic substances. There are two types of soil fertility viz.,
Inherent or Natural Fertility

The soil, as a nature contain some nutrients, which is known as inherent fertility. Among plant nutrients nitrogen, phosphorus and potassium is essential for the normal growth and yield of crop. The inherent fertility has a limiting factor from which the fertility is not decreased.
Acquired Fertility

The fertility develops by application of manures and fertilizers, tillage, irrigation, etc., is known as acquired fertility.
The acquired fertility has also a limiting factor. It is found by experiment that the yield does not increase remarkably by application of additional quantity of fertilizers.
Factors Effecting Soil Fertility

The factors that are effecting soil fertility may be of two types, i.e.,
Natural factors and
Artificial factors
The natural factors are those which influences the soil formation and the artificial factors are related to the proper use of land.
The factors effecting the fertility of soil are parent material, climate and vegetation, topography, inherent capacity of soil to supply nutrient, physical condition of soil, soil age, micro-organisms, availability of plant nutrients, soil composition, organic matter, soil erosion, cropping system and favourable environment for root growth.
Maintenance of Soil Fertility

Maintenance of soil fertility is a great problem of our farmers. Cultivation of particular crop year after year in the same field decreases the soil fertility. To increase the soil fertility, it is necessary to check the loss of nutrient and to increase the nutrient content of soil.
The following things must be properly followed for increasing the fertility of soil.
Proper use of land,
Good tillage,
Crop rotation,
Control of weeds,
Maintenance of optimum moisture in the soil,
Control of soil erosion,
Cultivation of green manure crops,
Application of manures,
Cultivation of cover crops,
Removal of excess water, (drainage)
Application of fertilizers,
Maintenance of proper soil reaction.


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Soil Reaction and Liming

It is well known fact that in high rainfall areas, due to the leaching of bases, acids soils are formed, while in low rainfall regions, on account of arid and semi arid conditions, saline and alkali soils occur.
Thus soil vary in acidity or alkalinity. The soil reaction is indicated by pH scale. When Ca(OH)2 or lime is added to the soil, it will become alkaline.
Liming of Acidic Soils

Liming means addition of any compound containing Calcium alone or both calcium and magnesium, that is capable of reducing the acidity of the soil. Lime correctly refers only to Calcium oxide (CaO), but the term as applied in agriculture is universally used to include various other materials also, like Calcium carbonate, Calcium hydroxide, Calcium - magnesium carbonate (marl) and Calcium silicate slags.
The effects of liming on the soil and plants are as follows :
Lime neutralizes soil acidity,
Beneficial soil bacteria are encouraged by adequate supplies of lime in the soil,
Lime makes phosphorus more available,
Liming helps the availability of potash and molybdenum,
Lime furnishes two essential elements, namely calcium and magnesium (if lime is dolamitic) for plant nutrition,
Lime reduces toxicity of Al, Mn and Fe,
Improves soil physical conditions.


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Fertilizers and Environmental Pollution

Fertilizers are relatively safer than pesticides which exhibit toxic properties on living systems. However, all the quantities of fertilizers applied to the soil are not fully utilized by plants. About 50 per cent of fertilizers applied to crops are left behind as residues. Though, inorganic fertilizers are not directly toxic to man and other life forms, they have been found to upset the existing ecological balance. The nutrients escape from the fields and are found in excessive quantities in rivers, lakes and coastal waters.
Algae blooms occur when the nutrient load is high, and these smother other aquatic vegetation and also interfere with the oxygen regulation in the water bodies. This phenomena may lead to loss of fish. Among the major synthetic plant nutrients, nitrogenous fertilizers cause most harm. Contamination of the environment arises because not all the fertilizer applied is taken up by the crop and removed at harvest. In tropical climate the maximum recovery in dry land crops is 50 to 60 per cent and 40 per cent in rice because much of nitrogen is lost as ammonia into the atmosphere.
Eutrophication of water bodies due to higher nitrate and phosphate concentrations, increasing levels of nitrates in drinking water sources, accumulation of heavy metals such as lead and cadmium in soils and water resources are the principal causes of environmental concerns due to fertilizer use in agriculture. In the a national wide survey it was found that many streams and more than 20 % of wells contain 10 to 50 mg or even more of nitrates per litre of water. The contamination is caused by domestic sewage leaking to the ground water. The nitrates in drinking water can lead to several ailments. Blue - baby syndrome in infants and gastric and other forms of cancer have been related with nitrates in drinking water or diet.
Another hazard associated with excessive use of fertilizers is the gaseous loss of nitrogen, into the atmosphere. High doses of carbon dioxide and ammonia that escape into the atmosphere both from fertilizer manufacturing plants and soils affect human health. Further the oxides of nitrogen have been reported to adversely affect the ozone layer, which protects the earth from UV radiation and heating up of earth.
The oxides of nitrogen cause respiratory diseases like asthma, lung cancer and bronchitis. Arsenic, ammonia are waste stream components of nitrogen manufacturing plants while fluoride, cadmium, chromium, copper, lead and manganese are waste stream components of phosphatic fertilizer industry. If these waste stream of components are not properly disposed they cause harm to human beings and animals with contamination of air and water.
The keeping quality of perishables like vegetables and fruits get declined with excess use of fertilizers particularly nitrogenous fertilizers.


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Economics of Fertilizer Use

Use of fertilizer by the farmer for increased crop production depends almost entirely on its economics. This is usually done by reporting response per unit area or per unit nutrient applied. With a view to convince the farmer about the profitability of fertilizer use, cost benefit ratio is also worked out.
Almost all such calculations are based on evaluating the extra produce at the support/market price and deducting the cost of fertilizer only at the statutory prevailing rates.
Due to high cost of commercial fertilizer marketed in India, the question of economics of fertilizer use has assumed great importance. The fertilizer association of India, New Delhi, therefore, organised series of group discussions on "Economics of Fertilizer use" during 1975. The recommendations of these group discussions are listed below,
Uniformity of approach in studying the economics of fertilizer is essential.
The fertilizer recommendations should be based on soil test values.
Balanced use of fertilizer should be advocated for better economic returns.
Use of nitrogenous fertilizer in split doses economises fertilizer use.
Micronutrient deficiencies should be corrected as and when needed.
Fertilizer schedule should be adopted for the whole crop sequence instead of a single crop.
To get the maximum benefit from the applied fertilizers, crops should be irrigated at the critical growth stages.